PNH Panel by FCM
Test Mnemonic
PNHPNL
CPT Codes
- 88184 - QTY (1)
- 88185 - QTY (2)
- 88187 - QTY (1)
LOINC ®
55164-8
Aliases
- Flow Panel for Paroxysmal Nocturnal Hemoglobinuria
Includes
- PNH Granulocyte clone
- PNH RBC Clone-Partial Ag Loss (Type II)
- PNH RBC Clone-Complete Ad Loss (Type III)
- Sum of PNH RBC Clones (Type II + Type III)
- Interpretation
- Reviewed by
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Analyte Specific Reagent
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 4 mL | Whole blood | EDTA (Lavender) | Ambient | Peripheral blood samples to be delivered to the flow cytometry lab within 24 hours of draw time. Samples greater than 48 hours old will be rejected. Do not draw on Fridays, weekends or holidays. | |
| 4 mL | Whole blood | EDTA (Lavender) | Refrigerated | Refrigerated | Peripheral blood samples to be delivered to the flow cytometry lab within 24 hours of draw time. Samples greater than 48 hours old will be rejected. Do not draw on Fridays, weekends or holidays. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | 48 hours |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
1 - 3 days
Methodology
| Name | Description |
|---|---|
| Flow Cytometry (FC) |
Reference Range
| PNH Panel by FCM | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative. No PNH clone detected. |
Special Info
Do not draw on Fridays, weekends or holidays. Specimens greater than 48 hours old will be rejected.
Clinical Info
The presence of paroxysmal nocturnal hemoglobinuria (PNH) clones in the erythrocyte and granulocyte populations is assessed in this procedure. For erythrocytes antibodies to Glycophorin A are used to specifically gate red cells and PNH clones are identified by lack of CD59 expression. For granulocytes, CD15 and CD 33 are used to specifically gate granulocytes. The PNH-type granulocytes are then identified by lack of expression of CD24 and lack of reactivity to Fluorescent Aerolysin (FLAER). The lower limit of detection for this assay is 0.01% PNH-type cells. The presence of a PNH clone occurs in classical hemolytic PNH, generally at levels above 1%. PNH clones may be seen in other disorders such as aplastic anemia and myelodysplastic syndrome. Thus, these results must be put in context of the clinical findings.