Non-variola orthopoxvirus (includes Mpox or monkeypox virus)
Test Mnemonic
OPXPCR
CPT Codes
- 87593 - QTY (1)
Aliases
- Vaccinia virus
- Non-variola orthopoxvirus
- Monkey
- Monkeypox
- Mpox
- Monkeypox Virus Qualitative PCR
Includes
- Orthopoxvirus Source
- Orthopoxvirus by PCR
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Swab | Fluid, lesion | Universal Transport Media (UTM) | Frozen | Specimen source required. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Swab | Fluid, lesion | Viral Transport Media | Frozen | Specimen source required. |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | 7 days |
| Frozen | 30 days |
| Ambient | 48 hours |
Days Performed
Mon, Wed, Fri
Turnaround Time
2 - 4 days
Methodology
| Name | Description |
|---|---|
| Qualitative Polymerase Chain Reaction |
Reference Range
| ORTHOPOXVIRUS BY PCR | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | Not Detected |
Special Info
Specimen source required. Calcium alginate swabs, wooden swabs and specimens without swabs will be rejected.
Clinical Info
This qualitative PCR test is used to detect members of the Orthopoxviruses, including monkeypox virus and vaccinia virus. This assay does not differentiate members of the Orthopoxviruses. Due to the 2022 global monkeypox outbreak, a detected result is most likely due to monkeypox virus. Identification of non-variola Orthopoxvirus DNA in an individual without suspicion for other recent Orthopoxvirus exposure meets the CDC definition for a “probable case” of monkeypox. Other Orthopoxviruses may be considered if appropriate.
Clinical Limitation
This test is intended for the detection of non-variola Orthopoxvirus DNA. A negative result does not rule out the presence of PCR inhibitors in the patient specimen or assay specific nucleic acid in concentrations below the level of detection by the assay. Clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status.