MGMT Methylation
Test Mnemonic
MGMT
CPT Codes
- 81287 - QTY (1)
Aliases
- MGMT
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| One | Paraffin block, formalin fixed | Formalin-fixed, paraffin-embedded block | N/A | Ambient | Minimal biopsy size 0.5 x 0.5 x 0.5 cm with mainly tumor tissue. Necrosis area should be less than 15%. Cut 12 charged, unbaked slides, 4um thick. Pre and post H&E slides must be examined. Tumor area will be microdissected from USS. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| One | Paraffin block, formalin fixed | Formalin-fixed, paraffin-embedded block | N/A | Ambient | Minimal biopsy size 0.5 x 0.5 x 0.5 cm with mainly tumor tissue. Necrosis area should be less than 15%. Cut 8 sections, 10 um thick and place in microcentrifuge tube. Pre and post H&E slides must be examined. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Ambient only is acceptable |
Days Performed
2 days per week
Turnaround Time
7 days
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) | |
| Pyrosequencing |
Reference Range
Special Info
Submit tissue specimen with anatomic pathology request form, and indicate MGMT Methylation on the form.
Clinical Info
Clinical predictive of response to temozolomide in patients diagnosed with glioblastomas.
Clinical Limitation
MGMT Methylation Assay detects methylation in Exon 1 of the MGMT gene. It does not detect methylation in the promoter.
Clinical Reference
Methylation not detected