Lymphocyte Transformation Test to Candida Antigen
Test Mnemonic
LTT
CPT Codes
- 86353 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 20 mL | Whole blood | Sodium heparin (Green) | Ambient | Ambient | Collect Monday - Thursday only. Deliver the specimen to the lab within 24 hours post collection. Do not aliquot. Specimen must remain at ambient temperature. Do not refrigerate. Do not freeze. Collect sample in a sterile container using aseptic technique. Sample cannot be shared with other testing. This test cannot be added on to other testing. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mL | Minimum volume only applies to pediatric patients; lower volumes may be considered on a case by case basis at the discretion of the medical director. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 24 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
10 - 14 days
Methodology
| Name | Description |
|---|---|
| Radioactive thymidine uptake in cell culture |
Reference Range
| Phytohemagglutinin | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | >=50.0 | SI | |||
| Ratio | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | >=2.0 | SI |
Special Info
Specimens should be sent only Monday thru Thursday by 4:00 pm. Specimen must remain at ambient temperature. Do not refrigerate. Do not freeze. Specimens will be cancelled if they arrive in lab greater than 24 hours post collection. Please call the lab to set-up order. If shipping specimen make sure it is in an ambient mailer and marked as "critical specimen".
Clinical Info
The test is used to assess lymphocytes proliferative response to Candida antigen. This is especially useful in patients suspected of having chronic mucocutaneous candidiasis. Clinical correlation is required. The test is reviewed and signed out by staff.