LPT to Beryllium, Blood
Test Mnemonic
BLDBE
CPT Codes
- 86353 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 80 mL | Whole blood | Sodium heparin (Green) | Ambient | Collect Monday - Wednesday only. Specimens must be delivered to lab by Thursday at 2pm each week; samples cannot be received on Fridays or Saturdays. Do not aliquot. Specimen must remain at ambient temperature. Do not refrigerate or freeze. Collect sample in a sterile container using aseptic technique. Sample cannot be shared with other testing. This test cannot be added on to other testing. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 30 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | Unacceptable |
| Frozen | Unacceptable |
Days Performed
Mon - Fri
Turnaround Time
10 - 14 days
Methodology
| Name | Description |
|---|---|
| Radioactive thymidine uptake in cell culture |
Reference Range
| Beryllium 1.0 uM D5 | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | < 3.0 | SI | |||
| Beryllium 1.0 uM D6 | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | < 3.0 | SI | |||
| Beryllium 10 uM D5 | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | < 3.0 | SI | |||
| Beryllium 10 uM D6 | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | < 3.0 | SI | |||
| Beryllium 100 uM D5 | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | < 3.0 | SI | |||
| PHA Stimulation Index | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | > 50.0 | SI |
Special Info
Contact testing laboratory at least 48 hours prior to collecting specimen at CellularImmunoPLMI@ccf.org. Specimen should not be collected or delivered on weekends or holidays.
Clinical Info
Lymphocyte proliferation test to Beryllium is a cellular assay used as an aid in diagnosing prior sensitization to Beryllium in the environment. Beryllium-sensitized individuals may remain asymptomatic for extended periods and never develop chronic berylliosis. Clinical, epidemiological, and radiological correlation is required. Bronchoalveolar lavage testing may offer higher sensitivity than whole blood testing where pulmonary disease is suspected.