Liver Fibrosis, FibroTest-ActiTest
Test Mnemonic
LIVFIB
CPT Codes
- 81596 - QTY (1)
LOINC ®
Not available
Aliases
- FibroTest
- Liver Acti
- Liver Fibrosis
- Necroinflammatory Activity Score
Includes
- Alanine aminotransferase (ALT)
- Alpha 2-Macroglobulin
- Apolipoprotein A1
- Bilirubin, Total
- Gamma-glutamyl Transferase (GGT)
- Haptoglobin
- Fibrosis Score
- Necroinflammat Act Score
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | SST (Gold) | Refrigerated | Centrifuge within 2 hours from collection. Protect serum from light after centrifugation. Overnight fasting is preferred. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Serum | No additive (Red) | Refrigerated | Centrifuge within 2 hours from collection. Transfer serum into a standard or amber aliquot tube. Protect serum from light after centrifugation. Overnight fasting is preferred. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 24 hours |
| Refrigerated | 7 days |
| Frozen | -20 C: Not acceptable; -70 C: 28 days |
Days Performed
Mon - Sat
Turnaround Time
2 - 5 days
Methodology
| Name | Description |
|---|---|
| Spectrophotometry (S) | |
| Colorimetric Enzyme Assay | |
| Immunoturbidometric Assay |
Reference Range
| Alpha 2 Macroglobulin | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Male | Normal | 80-290 | mg/dL | Female | Normal | 110-270 | mg/dL |
| ALT | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Male | Normal | 10-50 | U/L | Female | Normal | 10-35 | U/L |
| Apolipoprotein A1 | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| 2 Years | 17 Years | Normal | >120 | mg/dL | Male | 18 Years | 99 Years | Normal | >114 | mg/dL | Female | 18 Years | 99 Years | Normal | >124 | mg/dL |
| Bilirubin, Total | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.2-1.3 | mg/dL | |||
| Fibrosis Interpretation | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| FibroTest Score: >=0 and <=0.21 - Metavir Score: F0 no fibrosis | FibroTest Score: >0.21 and <=0.27 - Metavir Score: F0-F1 no fibrosis | FibroTest Score: >0.27 and <=0.31 - Metavir Score: F1 minimal fibrosis | FibroTest Score: >0.31 and <=0.48 - Metavir Score: F1-F2 minimal fibrosis | FibroTest Score: >0.48 and <=0.58- Metavir Score: F2 moderate fibrosis | FibroTest Score: >0.58 and <=0.72 - Metavir Score: F3 advanced fibrosis | FibroTest Score: >0.72 and <=0.74 - Metavir Score: F3-F4 advanced fibrosis | FibroTest Score: >0.74 and <=1.00- Metavir Score: F4 severe fibrosis |
| Fibrosis Score | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| No reference range available | |||||
| Fibrosis Stage | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| No reference range available | |||||
| GGT | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Male | Normal | 10-71 | U/L | Female | Normal | 6-42 | U/L |
| Haptoglobin | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 31-238 | mg/dL | |||
| Necroinflammatory Activity Grade | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| No reference range available | |||||
| Necroinflammatory Activity Interpretation | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| ActiTest Score: >=0 and <=0.17 - Metavir Score: A0 no activity | ActiTest Score: >0.17 and <=0.29 - Metavir Score: A0-A1 no activity | ActiTest Score: >0.29 and <=0.36 - Metavir Score: A1 minimal activity | ActiTest Score: >0.36 and <=0.52 - Metavir Score: A1-A2 minimal activity | ActiTest Score: >0.52 and <=0.60 - Metavir Score: A2 significant activity | ActiTest Score: >0.60 and <=0.62 - Metavir Score: A2-A3 significant activity | ActiTest Score: >0.62 and <=1.00 - Metavir Score: A3 severe activity |
| Necroinflammatory Activity Score | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| No reference range available |
Special Info
Protect from light. Avoid freeze/thaw cycles of specimen. Overnight fasting is preferred. Grossly hemolyzed and grossly lipemic specimens will be rejected. Specimens from patients less than 2 years old are unacceptable. Additionally, specimens QNS for one or more analytes will not be accepted.
Clinical Info
The FibroTest-ActiTest interpretation is a proprietary algorithm owned by BioPredictive. FibroTest and ActiTest permit the non-invasive evaluation of Hepatitis B or C individuals for the presence of liver fibrosis and liver inflammation, respectively. The FibroTest and ActiTest scores are calculated based on patient age, gender and concentrations of serum of y-glutamyl transferase (GGT), total bilirubin (TB), a-2 macroglobulin, haptoglobin, apolipoprotein A1 and alanine aminotransferase (ActiTest). Fibrotest and ActiTest Scores, on a scale of 0.0 to 1.0, are assigned a Metavir scale indicating the level of fibrosis or inflammation present.
Clinical Limitation
Specimens from patients less than 2 years of age are unacceptable.