Hemoglobin Electrophoresis
Test Mnemonic
HBELEC
CPT Codes
- 83020 - QTY (1)
- 83020(PC) - QTY (1)
Includes
- Hb A Percent
- Hb F Percent
- Hb A2 Percent
- Abnormal Hemoglobin
- Interpretation
- Reviewed by:
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 4 mL | Blood, peripheral | EDTA (Lavender) | Ambient | Refrigerated |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Unacceptable |
| Frozen | Unacceptable |
| Refrigerated | 7 days |
Days Performed
5 days per week
Turnaround Time
1 - 5 days
Methodology
| Name | Description |
|---|---|
| Capillary Electrophoresis (CE) |
Reference Range
| Hemoglobin A2 | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 2.0-3.1 | % | |||
| Hemoglobin Fetal | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 0.0-0.9 | % |
Special Info
If a variant hemoglobin is present, additional testing will be performed to identify the variant. Additional testing may include: Hemoglobin HPLC, acetate agar electrophoresis, cellulose acetate electrophoresis, sickle solubility, or isoelectric focusing.
Clinical Info
This test is designed for evaluation of suspected hemoglobin variants or beta thalassemia. If there is clinical suspicion for alpha thalassemia, suggest ordering "Hemoglobin Evaluation Cascade."