FISH for ROS1 Cyto Block
Test Mnemonic
ROS1CB
CPT Codes
- 88377 - QTY (1)
Includes
- LSI 6q22.1
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Analyte Specific Reagent
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | cytologic preparation (slide) | Slides | Ambient | Ambient | Cytology cell blocks that have been formalin fixed OR 4 μm formalin fixed paraffin-embedded tissue sections on electrostatically charged slides containing representative tumor, including Pre-H&E. Samples must not be fixed in Prefer or B5. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Cytology cell blocks that have been formalin fixed OR 4 μm formalin fixed paraffin-embedded tissue sections on electrostatically charged slides containing representative tumor, including Pre-H&E. Samples must not be fixed in Prefer or B5. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Cell block slides are acceptable |
| Refrigerated | Not preferred |
| Frozen | Unacceptable |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Special Info
This assay has been validated for cell blocks prepared from cytology samples fixed in PreservCyt solution using the Cellient Automated Cell Block System (Hologic, Marlborough, MA). Blocks prepared with other fixatives and/or methods are not acceptable.
Clinical Info
Interphase FISH analysis utilizing a dual color, break-apart probe for the ROS1 gene at 6q22.1. Rearrangements involving ROS1 may be seen in individuals with late-stage, lung adenocarcinomas that are negative for EGFR and ALK rearrangements. The translocation partner gene cannot be identified with this assay. If no cells are available for analysis, no analysis charges will be incurred.