FISH for IGH/BCL2 Tissue
Test Mnemonic
T1418
CPT Codes
- 88377 - QTY (2)
Aliases
- FISH for t(14;18)
Includes
- LSI 14q32.33
- LSI 18q21.33
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Slide (s), unstained | Clean container | Ambient | Ambient | 4 μm tissue sections on electrostatically charged slides containing representative tissue. Tissue must be formalin fixed. Samples must not be fixed in B5 or Prefer fixative. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Blocks and slides should be transported at ambient temperature. |
Days Performed
5 days per week
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Interphase FISH analysis utilizing a dual color, dual fusion probe set specific to the IGH gene at 14q32.33 and the BCL2 gene at 18q21.33. This is useful in identifying the t(14;18) translocation present in follicular lymphoma and diffuse large B-cell lymphoma. If no cells are available for analysis, no analysis charges will be incurred.