FISH for EGFR
Test Mnemonic
EGFRF
CPT Codes
- 88377 - QTY (1)
Aliases
- EGFR Amplification
- Epidermal Growth Factor Receptor by FISH.
Includes
- FISH for 7p12
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Slide (s), unstained | Clean container | Ambient | One formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | FFPE tissue blocks and slides from FFPE specimens are stable at ambient temperatures |
Days Performed
5 days per week
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Interphase FISH analysis utilizing an enumeration probe for the EGFR gene at 7p11.2, as well as a probe specific to the centromere of chromosome 7, useful in the diagnosis of astrocytomas, oligodendrogliomas, ependymomas, or mixed gliomas. If no cells are available for analysis, no analysis charges will be incurred.