FISH for Aggressive B-cell Lymphoma
Test Mnemonic
FABCEL
CPT Codes
- 88377 - QTY (4)
Aliases
- FABCEL
- FISH for Aggressive B-Cell Lymphoma (Tissue)
Includes
- FISH for BCL2 Translocations
- FISH for BCL6
- FISH for c-MYC (8q24)
- FISH for t(8;14)(q24;32)
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 6 | Slide (s), unstained | Clean container | Ambient | Ambient | One formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Sample is formalin fixed and must not be fixed in Prefer or B5 |
Days Performed
5 days per week
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Interphase FISH analysis used to assist in the diagnosis of various aggressive B-cell lymphomas. A panel of four FISH probes are employed: a dual color MYC break-apart probe, a dual fusion, dual color MYC::IGH probe, a dual color BCL2 break-apart probe, and a dual color BCL6 break-apart probe. The translocation partner gene(s) cannot be identified in this assay. If no cells are available for analysis, no analysis charges will be incurred.