Entamoeba histolytica, IgG
Test Mnemonic
EHISTO
CPT Codes
- 86753 - QTY (1)
LOINC ®
9522-4
Aliases
- Amebiasis Antibodies
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | SST (Gold) | Refrigerated | Separate serum from cells ASAP or within 2 hours of collection. Please mark specimen plainly as ACUTE or CONVALESCENT. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: 2 days |
| Refrigerated | After separation from cells: 2 weeks |
| Frozen | After separation from cells: 1 year (Avoid repeated freeze/thaw cycles) |
Days Performed
Tue, Fri
Turnaround Time
3 - 7 days
Methodology
| Name | Description |
|---|---|
| Enzyme-Linked Immunosorbent Assay (ELISA) |
Reference Range
| Entamoeba histolytica, IgG | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative: No significant level of detectable E. histolytica IgG Antibody: 8 U or less | Equivocal: (Repeat testing in 10-14 days may be helpful) 9 - 11 U | Positive: (Current or past infection) 12 U or greater |
Special Info
Acute and convalescent specimens must be labeled as such; parallel testing is preferred and convalescent specimens MUST be received within 30 days from receipt of the acute specimens. Unacceptable conditions: Contaminated, heat-inactivated, hemolyzed or severely lipemic specimens. This test is New York DOH approved.
Clinical Info
Aid in the detection of amebic liver abscess. Test is not useful for intestinal infection. In case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative. Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.