Cryoglobulin, Qualitative




Test Mnemonic

CRYO

CPT Codes

  • 82595 - QTY (1)

Performing Laboratory

Cleveland Clinic Laboratories

FDA Category

Analyte Specific Reagent


Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
3 mLSerumNo additive (Red)N/ASee noteDo not use serum separator tubes. Keep sample warm until separated from cells. Immediately after collection, place tubes in heel warmer or 37 degree C (warm, not hot) water. Allow to clot at 37 degrees C for 90 minutes. Centrifuge at 37 C if possible (do not use refrigerated centrifuge), and separate serum from cells. Refrigerate serum after separation from cells.

Minimum Specimen Requirements

Volume Type Container Collect Temperature Transport Temperature Special Instructions
1 mL     

Stability

Environmental Condition Description
Ambient24 hours
Refrigerated45 days
FrozenUnacceptable

Days Performed

Wed

Turnaround Time

4 - 10 days

Methodology

Name Description
Visual 

Reference Range

Special Info

1. Pre-warm tube(s) to 37°C in a water bath, incubator, or in a cup with heal warmers. 2. Immediately after collection, incubate the clotting blood for 90 minutes in a 37°C water bath, incubator, or in a cup with heal warmers. 3. Centrifuge tube(s) for 5 minutes at 3,000 rpm at room temperature 4. Remove the serum using a polystyrene transfer pipette and place into a labelled plastic tube. If needed repeat step 3 to remove extraneous red blood cells. 5. Transfer serum into a second properly labeled plastic tube. Store at 2-8°C for at least 72 hours to precipitate the cryoglobulins. Slight to moderate hemolysis is acceptable.

Clinical Info

To detect circulating immune complexes and other cryoprecipitate para-proteins.

Clinical Limitation

Patients should fast for 8h before sample collection to minimize the potential for turbidity due to triglycerides.