BCR/ABL1 Mutation Analysis for Tyrosine Kinase Inhibitor Resistance by NGS
Test Mnemonic
KINASE
CPT Codes
- 81170 - QTY (1)
Performing Laboratory
ARUP
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 5 mL | Whole blood | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3 mL | Bone marrow | EDTA (Lavender) | Refrigerated | Critical refrigerated. Specimen must be delivered to Cleveland Clinic Laboratories by 3 p.m. DO NOT collect the day before or the day of a major holiday. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | 48 hours |
| Ambient | 1 hour |
| Frozen | Unacceptable |
Days Performed
Varies
Turnaround Time
11 - 13 days
Methodology
| Name | Description |
|---|---|
| Massive Parallel Sequencing |
Reference Range
Special Info
Serum, plasma, and specimens collected in anticoagulants other than EDTA are unacceptable. Frozen, clotted or severely hemolyzed specimens will be rejected. For specimens having no t(9;22) fusion, this test will be canceled, and an ABL1 amplification confirmation test will be ordered. This test is New York DOH approved.
Clinical Info
Order only for patients with an established diagnosis of a BCR-ABL1 positive leukemia. Used to determine if a mutation is present that would interfere with response to TKI therapy in Philadelphia chromosome positive (Ph+) lymphoblastic leukemia or chronic myelogenous leukemia (CML). This test detects all common mutations, including T315I.