FISH for DDIT3/GLI1(12q13.3)
Test Mnemonic
DDIT3
CPT Codes
- 88271 - QTY (2)
- 88275 - QTY (1)
- 88291 - QTY (1)
Aliases
- GLI1
- MLS
- Myxoid Liposarcoma
- DDIT3/GLI1
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Slide (s), unstained | Ambient | Ambient | One formalin-fixed, paraffin embedded block containing representative tissue OR unstained 4 μm tissue sections on electrostatically charged slides containing representative tumor. Tissue must be fixed in formalin NOT B5 or PREFER fixative |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Blocks/Slides stable at ambient temperatures. |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Interphase FISH analysis utilizing a dual color, break-apart probe for the DDIT3/GLI1 genes at 12q13.3. Rearrangements involving the DDIT3 (CHOP) gene are observed in myxoid/round cell liposarcoma. The translocation partner gene cannot be identified in this assay. This assay also detects GLI1 amplification, which may be seen in mesenchymal soft tissue neoplasms. If no cells are available for analysis, no analysis charges will be incurred.