FISH for PDGFRA Bone Marrow
Test Mnemonic
PGFRAM
CPT Codes
- 88271 - QTY (3)
- 88275 - QTY (1)
- 88291 - QTY (1)
- 88237 - QTY (1)
Aliases
- FISH for 4q12
Includes
- FISH for PDGFRA (4q12)
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | EDTA (Lavender) | Ambient | Ambient |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | Sodium heparin (Green) | Ambient | Ambient |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | Acceptable |
| Frozen | Not acceptable |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
This assay employs a tri-color probe to detect rearrangements of the PDGFRA locus on chromosome 4q12. Myeloid neoplasms with PDGFRA rearrangements are often associated with eosinophilia and represent a distinct clinicopathologic entity in the WHO classification of hematolymphoid neoplasms. This assay is useful for the evaluation of unexplained eosinophilia and to assist in the diagnosis and classification of myeloproliferative neoplasms. If no cells are available for analysis, no analysis charges will be incurred.