FISH for ALK Cyto Block
Test Mnemonic
ALKCB
CPT Codes
- 88377 - QTY (1)
- 88377PC - QTY (1)
Includes
- FISH for 2p23.2-p23.1
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | cytologic preparation (slide) | Ambient | Ambient | Cytology cell blocks that have been formalin fixed OR 4 μm formalin fixed paraffin-embedded tissue sections on electrostatically charged slides containing representative tumor, including Pre-H&E. Samples must not be fixed in Prefer or B5. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Cytology cell blocks that have been formalin fixed OR 4 μm formalin fixed paraffin-embedded tissue sections on electrostatically charged slides containing representative tumor, including Pre-H&E. Samples must not be fixed in Prefer or B5. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Cell block slides are acceptable |
| Refrigerated | Not preferred |
| Frozen | Unacceptable |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Special Info
This assay has been validated for cell blocks prepared from cytology samples fixed in formalin ONLY. Blocks prepared with other fixatives and/or methods are not acceptable
Clinical Info
Interphase FISH analysis utilizing a dual color, break-apart probe for the ALK gene at 2p23.2. Rearrangements involving ALK may be seen in individuals with late-stage, non-small lung cell cancer who may benefit from specific drug treatment. The translocation partner gene cannot be identified in this assay. If no cells are available for analysis, no analysis charges will be incurred.