FISH for 8;21 Translocation for AML Bone Marrow
Test Mnemonic
AMLFBM
CPT Codes
- 88237 - QTY (1)
- 88271 - QTY (2)
- 88275 - QTY (1)
- 88291 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Analyte Specific Reagent
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | EDTA (Lavender) | Ambient | Ambient |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | Sodium heparin (Green) | Ambient | Ambient |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | Acceptable |
| Frozen | Not acceptable |
Days Performed
4 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
Used in the diagnosis of Acute Myelogenous Leukemia. FISH analysis with the AML1 and ETO probes is valuable in cases of AML M2 because of its ability to reveal masked or variant, t(8;21) (q22;q22) translocations.