FISH for IGH/BCL2 Bone Marrow
Test Mnemonic
FSFCLM
CPT Codes
- 88271 - QTY (2)
- 88275 - QTY (1)
- 88291 - QTY (1)
- 88237 - QTY (1)
Aliases
- FISH for t(14;18) translocation
Includes
- LSI IGH (14q32)
- LSI BCL2 (18q21)
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | EDTA (Lavender) | Ambient | Refrigerated |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 - 3 mL | Bone marrow | Sodium heparin (Green) | Ambient | Refrigerated |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 48 hours |
| Refrigerated | Acceptable |
| Frozen | Not acceptable |
Days Performed
5 days per week
Turnaround Time
3 - 5 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Clinical Info
This assay uses a dual color, dual fusion probe to detect the IGH/BCL2 translocation that is associated with follicular lymphoma and diffuse large B-cell lymphoma. This test should not be used to monitor minimal residual disease. If no cells are available for analysis, no analysis charges will be incurred.