Methadone Quantitation, Urine
Test Mnemonic
UQMET
CPT Codes
- G0480 - QTY (1)
- 80358 - QTY (1)
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 10 mL | Urine, random | Clean container (No preservatives) | Refrigerated |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 3 days |
| Refrigerated | 2 weeks |
| Frozen | 2 weeks |
Days Performed
4 days per week
Turnaround Time
2 - 5 days
Methodology
| Name | Description |
|---|---|
| High Performance Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) |
Reference Range
| EDDP, Urine | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| < 6 ng/mL | |||||
| Methadone, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| < 16 ng/mL | |||||
| Specimen Validity Chromate, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | <50 | mg/L | |||
| Specimen Validity Creatinine, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Years | Years | Normal | 20.0-300.0 | mg/dL | |
| Specimen Validity Nitrite, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | <500 | mg/L | |||
| Specimen Validity Oxident, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | <200 | mg/L | |||
| Specimen Validity pH, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 4.5 - 8.0 | ||||
| Specimen Validity Specific Gravity, Urine | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | 1.003 - 1.035 |
Special Info
For medical purposes only; not valid for forensic use. Specimen Validity Testing (SVT) analytes have a stability of up to 5 days at 2-8°C as stated in the IFU. Based on the laboratory’s studies, this stability is based on samples that have reached an equilibrium. Introduction of oxidants to a urine sample will not be stable until an equilibrium is achieved. Time to reach equilibrium will vary dependent on the type and amount of oxidant used as an adulterant.
Clinical Info
For Specimen Validity Interpretations, the following rules are applied: Diluted: Creatinine is greater than or equal to 2mg/dL but less than 20mg/dL and Specific Gravity is greater than 1.0010 but less than 1.0030. Substituted: Creatinine is less than 2mg/dL and the Specific gravity is less than or equal to 1.0010 or greater than or equal to 1.0200. Adulterated: pH is less than 4 or greater than or equal to 11; Nitrite is greater than or equal to 500mg/L; Chromate is greater than or equal to 50mg/L; Oxidant is greater than or equal to 200mg/L.