FISH for ROS1 (6q22)
Test Mnemonic
ROS1
CPT Codes
- 88377 - QTY (1)
Aliases
- ROS1 gene rearrangement
- ROS1 gene rearrangement by FISH
Includes
- LSI 6q22.1
Performing Laboratory
Cleveland Clinic Laboratories
FDA Category
Analyte Specific Reagent
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Paraffin block, formalin fixed | Slides | Ambient | Ambient | Pre and post H&E with two unstained slides |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 slides | Pre and post H&E with two unstained slides |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Slides from FFPE Tissue at room temp |
Days Performed
5 days per week
Turnaround Time
5 - 7 days
Methodology
| Name | Description |
|---|---|
| Fluorescent In-Situ Hybridization (FISH) |
Reference Range
Special Info
Pre and post H&E with two unstained slides
Clinical Info
Interphase FISH analysis utilizing a dual color, break-apart probe for the ROS1 gene at 6q22.1. Rearrangements involving ROS1 may be seen in individuals with late-stage, lung adenocarcinomas that are negative for EGFR and ALK rearrangements. The translocation partner gene cannot be identified with this assay. If no cells are available for analysis, no analysis charges will be incurred.
Clinical Reference
Shaw AT, Sai-Hong IOU, Yung-Jue B, et al. Crizotinib in ROS1-Rearranged Non–Small-Cell Lung. National Comprehensive Cancer Network (NCCN) Clinical Practice Guidelines in Oncology, Non-Small Cell Lun