HIV-1 RNA, Qualitative, TMA
Test Mnemonic
HIVTMA
CPT Codes
- 87535 - QTY (1)
Includes
- HIV-1 Qualitative by NAAT
Performing Laboratory
ARUP
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | EDTA (Lavender) | Frozen | Separate plasma from cells ASAP or within 24 hours of collection, transfer into standard aliquot tube and freeze. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 2 mL | Plasma | ACD A or B (Yellow) | Frozen | Separate plasma from cells ASAP or within 24 hours of collection, transfer into standard aliquot tube and freeze. | |
| 2 mL | Plasma | EDTA PPT (White) | Frozen | Separate plasma from cells ASAP or within 24 hours of collection, transfer into standard aliquot tube and freeze. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.8 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Refrigerated | After separation from cells: 72 hours |
| Frozen | After separation from cells: 90 days |
| Ambient | After separation from cells: Unacceptable |
Days Performed
Sun - Sat
Turnaround Time
2 - 5 days
Methodology
| Name | Description |
|---|---|
| Transcription-Mediated Amplification |
Special Info
Improper specimen handling can cause false negatives or contamination.
Clinical Info
Reference Interval: Not detected. This test detects human immunodeficiency virus type 1 (HIV-1) RNA from Group M, N and O subtypes; it does not detect HIV-1 proviral DNA. A result of "Not Detected" does not rule out HIV-1 RNA concentrations below the limit of detection of the assay or the presence of inhibitors in the patient specimen. The diagnosis of HIV-1 infection should not be made based solely on a single HIV-1 test result. Diagnosis requires repeat and confirmatory testing as recommended by U.S. Health and Human Services Guidelines. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).