Complement, Alternate Pathway (AH50), Functional
Test Mnemonic
COMAP
CPT Codes
- 86161 - QTY (1)
Aliases
- AH50
Performing Laboratory
ARUP
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Serum | No additive (Red) | Frozen, Critical | CRITICAL FROZEN (-70°C). Locations without a -70°C freezer should not collect this test. Do not use gel separator tubes. Allow specimen to clot for 1 hour at room temperature. Centrifuge (at refrigerated temperature if possible) and separate serum from cells ASAP or within 2 hours of collection. Transfer into standard aliquot tube and freeze immediately in a -70°C freezer. Separate specimens must be submitted when multiple tests are ordered. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.3 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Frozen | After separation from cells: 30 days at -70 degrees C (Avoid multiple freeze/thaw cycles) |
| Ambient | After separation from cells: Unacceptable |
| Refrigerated | After separation from cells: Unacceptable |
Days Performed
Sun, Wed
Turnaround Time
8 - 15 days
Methodology
| Name | Description |
|---|---|
| Enzyme-Linked Immunosorbent Assay (ELISA) |
Reference Range
| Complement, Alternate Pathway (AH50), Functional | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Normal | >= 31 | % normal |
Special Info
CRITICAL FROZEN. Locations without a -70°C freezer should not collect this test. Do not use gel separator tubes. Separate specimens must be submitted when multiple tests are ordered. Unacceptable conditions include specimen types other than serum, specimens left to clot at refrigerated temperature, specimens exposed to repeated freeze/thaw cycles, or specimens that are grossly hemolyzed, lipemic or icteric.
Clinical Info
This test is useful as initial screening for suspected deficiency in the alternative complement pathway.