Universal PCR, Acid Fast Bacilli
Test Mnemonic
AFBPCR
CPT Codes
- 87551 - QTY (1)
- 87556 - QTY (1)
Aliases
- Universal Acid Fast Bacilli PCR
- Universal AFB PCR
Performing Laboratory
University of Washington Molecular Microbiology
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Fluid, body | Sterile container | Frozen |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| Other | Tissue, fresh or frozen | Sterile container | Frozen | Place 1 cubic cm or less of tissue in a sterile, DNA-free container and ship on dry ice. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 0.2 mL | For Body Fluid |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | Paraffin block: Indefinitely; Tissue: 6 hours |
| Refrigerated | Unacceptable (Tissue: 7 days with disclaimer) |
| Frozen | 30 days |
Days Performed
Mon - Fri
Turnaround Time
1 - 2 weeks
Methodology
| Name | Description |
|---|---|
| Polymerase Chain Reaction (PCR) | |
| Sequencing |
Reference Range
Special Info
ICD9/Diagnosis codes preferred, but not required. If tissue specimen is held at any condition other than frozen as described in the stability section, add a note to the test order so that the appropriate disclaimer can be added. Swabs, direct blood, serum, plasma, and stool specimens will be rejected. Tissues floating in excess formalin will be rejected.
