Mycoplasma Culture
Test Mnemonic
MYPLAS
CPT Codes
- 87109 - QTY (1)
LOINC ®
6486-5
Aliases
- Culture, Mycoplasma
- Mycoplasma hominis Culture
- Ureaplasma urealyticum Culture
Performing Laboratory
Cleveland Clinic Laboratories
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| One | Cervical | Swab in Universal Transport Media (UTM) | Frozen | Transport media must be inoculated at point of collection. UTM is the preferred transport and available from the storeroom (Lawson #386421). M4 is acceptable as an alternative. | |
| One | Endocervical | Swab in Universal Transport Media (UTM) | Frozen | Transport media must be inoculated at point of collection. UTM is the preferred transport and available from the storeroom (Lawson #386421). M4 is acceptable as an alternative. | |
| One | Urethral | Swab in Universal Transport Media (UTM) | Frozen | Transport media must be inoculated at point of collection. UTM is the preferred transport and available from the storeroom (Lawson #386421). M4 is acceptable as an alternative. | |
| One | Urogenital | Swab in Universal Transport Media (UTM) | Frozen | Transport media must be inoculated at point of collection. UTM is the preferred transport and available from the storeroom (Lawson #386421). M4 is acceptable as an alternative. | |
| One | Vaginal | Swab in Universal Transport Media (UTM) | Frozen | Transport media must be inoculated at point of collection. UTM is the preferred transport and available from the storeroom (Lawson #386421). M4 is acceptable as an alternative. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1 mL | Semen | Transport Medium | Frozen | Place 1 mL of specimen in Universal Transport Media (UTM) (available through storeroom Lawson #386421). Transport media must be inoculated at point of collection. UTM is preferred; M4 is acceptable as an alternative. | |
| 1 mL | Urine, random | Transport Medium | Frozen | Place 1 mL of specimen in Universal Transport Media (UTM) (available through storeroom Lawson #386421). Transport media must be inoculated at point of collection. UTM is preferred; M4 is acceptable as an alternative. |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | 8 hours |
| Refrigerated | In UTM or M4: 48 hours Urine, semen or body fluids not in transport medium < 24 hours |
| Frozen | In UTM or M4: 1 month Urine, semen or body fluids not in transport medium < 24 hours |
Days Performed
Sun - Sat
Turnaround Time
10 days
Methodology
| Name | Description |
|---|---|
| Culture |
Reference Range
| Mycoplasma Culture | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Negative for Mycoplasma hominis or Ureaplasma urealyticum (The Mycofast test detects Mycoplasma hominis at levels >10,000 CFU/ml and Ureaplasma urealyticum at levels >1,000 CFU/ml) |
Special Info
Adherence to proper specimen collection, transport guidelines and temperature are critical to isolation of the organisms. Transport media must be inoculated at the point of collection. Dry swabs, swabs in bacterial transport media or swabs in M4 RT will be rejected.
Clinical Info
This test is designed for rapid detection and identification of Ureaplasma urealyticum and Mycoplasma hominis from genitourinary specimens. Although these organisms represent normal flora of the urogenital tract, disease associations include urethritis and infertility. Vertical transmission during pregnancy or at delivery may result in colonization or neonatal infection (pneumonia, bacteremia, meningitis). Specimens from nongenital sites will be sent to a reference laboratory (order UMPLAS). Note: For detection of Mycoplasma pneumoniae from respiratory sites, refer to the Mycoplasma pneumoniae PCR test.