Barbiturates
Test Mnemonic
BARBS
CPT Codes
- 80345 - QTY (1)
- G0480 - QTY (1)
Includes
- Butalbital
- Pentobarbital
- Phenobarbital
Performing Laboratory
ARUP
FDA Category
Laboratory Developed Test
Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3.5 mL | Plasma | Potassium oxalate/sodium fluoride (Gray) | Refrigerated | Do not use separator tubes. Separate plasma from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. |
Alternate Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 3.5 mL | Plasma | EDTA (Lavender) | Refrigerated | Do not use separator tubes. Separate plasma from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. | |
| 3.5 mL | Serum | No additive (Red) | Refrigerated | Do not use separator tubes. Separate serum from cells ASAP or withing 2 hours of collection and transfer into standard aliquot tube. | |
| 3.5 mL | Plasma | Sodium heparin (Green) | Refrigerated | Do not use separator tubes. Separate plasma from cells ASAP or within 2 hours of collection and transfer into standard aliquot tube. |
Minimum Specimen Requirements
| Volume | Type | Container | Collect Temperature | Transport Temperature | Special Instructions |
|---|---|---|---|---|---|
| 1.5 mL |
Stability
| Environmental Condition | Description |
|---|---|
| Ambient | After separation from cells: 1 week |
| Refrigerated | After separation from cells: 2 weeks |
| Frozen | After separation from cells: 3 years (Avoid repeated freeze/thaw cycles) |
Days Performed
Tue, Thu, Sat
Turnaround Time
2 - 8 days
Methodology
| Name | Description |
|---|---|
| Gas Chromatography Mass Spectrometry (GCMS) | |
| Quantitative Liquid Chromatography - Tandem Mass Spectrometry |
Reference Range
| Butalbital | |||||
|---|---|---|---|---|---|
| Sex | Age From | Age To | Type | Range | Range Unit |
| Cut off: 50 ng/mL | |||||
| Pentobarbital | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Cut off: 50 ng/mL | |||||
| Phenobarbital | |||||
| Sex | Age From | Age To | Type | Range | Range Unit |
| Cut off: 50 ng/mL |
Special Info
All drugs will be quantified if positive. Positive cutoff for all drugs: 50 ng/mL. Separator tubes will be rejected. Hemolyzed specimens will be rejected. Specimens exposed to repeated freeze/thaw cycles are unacceptable. Plasma or whole blood collected in sodium citrate (light blue) tubes will be rejected. This test is a reflex from Drug Screen 9 Panel, Serum or Plasma (DRGSC9). This test is New York DOH approved.
Clinical Info
This test can be useful to optimize drug therapy, monitor patient adherence, or rule out barbiturate exposure. The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing.
Clinical Limitation
For medical purposes only; not valid for forensic use.