Complement Deficiency Assay | Cleveland Clinic Laboratories

Complement Deficiency Assay Print

Test Mnemonic

COMPD

CPT Codes

86162 - QTY (1)

Aliases

CH50
Total Complement Function Test

Performing Laboratory

Cleveland Clinic Laboratories

Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
1 mL	Serum	No additive (Red)		Frozen	Allow sample to clot, separate serum from cells as soon as possible. Centrifuge, then remove serum and freeze at -20 or colder (-70 or colder is preferred).

Minimum Specimen Requirements

Volume	Type	Container	Collect Temperature	Transport Temperature	Special Instructions
0.25 mL

Stability

Environmental Condition	Description
Frozen	7 days at -20 °C (acceptable); 30 days at -70 °C (preferred)
Refrigerated	24 Hours
Ambient	4 Hours

Days Performed

Mon, Thu

Turnaround Time

1 - 5 days

Methodology

Name	Description
Turbidimetric Immunoassay (TUI)

Reference Range

Complement Deficiency Assay
Sex	Age From	Age To	Type	Range	Range Unit
			Normal	41.7-95.1	U/mL

Special Info

Samples must be collected in a red-top tube, without serum separator. If frozen, samples should be kept at -20 or colder (-70 or colder is preferred).

Clinical Info

The test is used as an aid to assess the functional performance of the classical pathway of the complement system. Clinical correlation is required. This assay is performed on the Binding Site Optilite turbidimetric analyzer.

Clinical Limitation

Sera must be handled properly to prevent in vitro complement activation. Sample should not be frozen and thawed more than three times. This assay has not been evaluated for the pediatric population. Turbidimetric assays are not suitable for the measurement of highly lipemic or hemolyzed samples or samples containing high levels of circulating immune complexes due to the unpredictable degree of non-specific scatter these sample types may generate.